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ASU Team Develops Quick Way to Determine Bacteria’s Antibiotic Resistance

September 4, 2015

Staphylococcus_aureus_VISA_2Bacteria’s ability to become resistant to antibiotics is a growing issue in health care: Resistant strains result in prolonged illnesses and higher mortality rates.

One way to combat this is to determine bacteria’s antibiotic resistance in a given patient, but that often takes days — and time is crucial in treatment. ASU scientists have developed a technique that can sort antibiotic-resistant from “susceptible” bacteria, and it happens in a matter of minutes.

The microfluidic technology, developed in the lab of professor Mark Hayes in the Department of Chemistry and Biochemistry at Arizona State University, uses microscale electric field gradients, acting on extremely small samples, to tell the difference between the two strains (antibiotic-resistant and antibiotic-susceptible) ofStaphylococcus epidermidis.

It’s part of a changing approach to bacteria.

After a multi-decade “sledgehammer” approach, focused on killing all bacteria via soaps, detergents, antibiotics and hand sanitizers, scientists are now moving to subtler methods based on a better understanding of the complex bacterial system in our bodies and in the world around us.

The average human has more than 100 trillion microbes in and on his/her body. That’s nine times the number of cells that make up the entire human body. Armies of bacteria sneak into our bodies the moment we are born, uninvited but necessary guests. We can’t do without them.

Bacteria have, by evolution, the ability to rapidly adopt beneficial genomic alterations. One type of adaptation is resistance to antibiotics, and this is becoming a huge and worldwide problem.

National summary data from the Centers for Disease Control and Prevention indicate that each year in the United States, at least 2 million people acquire serious infections with antibiotic-resistant strains of bacteria. At least 23,000 people die as a direct result of these infections, and many more die from related complications.

It is not just humans that are threatened by this growing adaptation. A study reported in Science just last week finds a wide variety of African wildlife harbor drug-resistant microbes similar to those found in humans living in nearby villages.

Antibiotic-resistance adaptation is a natural phenomenon. When an antibiotic is used, bacteria that can resist that antibiotic obviously have a greater chance of survival than those that are susceptible, and so the resistance passes to the next generation.

Some of the most notorious antibiotic-resistant strains and species belong to the genus Staphylococcus. They are typically classified as pathogenic or non-pathogenic based on production of the enzyme coagulase. Staphylococcus epidermidis does not produce coagulase, and it is generally less invasive than Staphylococcus aureus. In fact, it is a normal and commensal resident of human skin.

In recent decades, however, Staphylococcus epidermidis has increasingly emerged as a cause of multi-resistant hospital-acquired infections. Immunocompromised patients, indwelling medical devices, and surgically implanted prostheses provide suitable environments for Staphylococcus epidermidis to propagate and form biofilms.

This is where the project began, as a collaboration with orthopedic surgeon Dr. Alex McLaren and his team member and bioengineer Dr. Ryan McLemore of Banner Good Samaritan Medical Center, Phoenix, along with Dr. Mark Spangehl of the Mayo Clinic College of Medicine, Arizona.

By most metrics the antibiotic-resistant and susceptible strains of Staphylococcus epidermidis are phenotypically identical, and thus present a major challenge to traditional analytical separation techniques. This is where the ASU technology is finding its place.

Current clinical approaches to determination of antibiotic resistance often require two or more days to obtain results. They typically rely upon treating the bacteria with antibiotics, then observing colony growth patterns. The long turnaround times lead to increased reliance upon broad-spectrum antimicrobials and generally lead to suboptimal outcomes for patients (including increased mortality rates).

Scientists in the Hayes group at ASU’s Department of Chemistry and Biochemistry — soon to become the School of Molecular Sciences — are enabling a handheld, battery-operated device that might deliver answers in minutes, instead of days.

Read the full article and findings at Arizona State University



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